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We also present quality assessment reports for rnaSPAdes assemblies, compare it with modern transcriptome assembly tools using several evaluation approaches on Information about SPAdes download, requirements, installation and basic options can be found in SPAdes manual. Below rnaSPAdes is a tool for de novo transcriptome assembly from RNA-Seq data and is suitable installation and basic options can be found in SPAdes manual. --rna (same as rnaspades.py ) This flag should be used when assembling RNA-Seq data sets (runs rnaSPAdes). To learn more, see rnaSPAdes manual. User manual. Links. galaxy.pasteur.fr/tool_runner?tool_id=toolshed.pasteur.fr/repos/fmareuil/rnaspades/rnaspades/3.9.0 Galaxy service. command. rnaspades.py [options] -o . blreads. Assemble --> rnaspades. Documentation. User's guide. $doc/spades/rnaspades_manual.html. Manual. rnaSPAdes is a tool for de novo transcriptome assembly from RNA-Seq data and is suitable for all kind of organisms. rnaSPAdes is a part of SPAdes package since