Fermentas pfu dna polymerase manual

A family B DNA polymerase (KOD DNA polymerase) was found in this KOD1 strain and shows various unique properties (1). 4. Comparison of elongation rates of KOD Pfu and Taq DNA polymerase. The elongation rate was measured according to the length of synthesized DNA, using Pfu DNA polymerase Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus, where it functions in Pfu DNA polymerase is hence superior for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to DNA polymerases used to amplify targets during PCR cloning are high fidelity enzymes with error frequencies typically in the range of. All enzymes and reaction buffers were from commercial sources: Fermentas (Taq polymerase), Invitrogen/Life Technologies (AccuPrime-Taq), EMD Several DNA polymerase variants with increased DNA replication fidelity are known[54], e.g. for the Klenow fragment of E. coli DNA polymerase I and the thermostable Thermus aquaticus (Taq) DNA polymerase[55] or the Pyrococcus furiosus (Pfu) DNA polymerase[56]. Recombinant Pfu DNA Polymerase; supplied with 10X Reaction Buffer. PFU DNA Polymerase 500 units. Add to cart to purchase or to request a quote. Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus, where it functions to copy the organism's DNA during cell division. Pfu DNA polymerase has superior thermostability and proofreading properties compared to Taq DNA polymerase. Pfu DNA polymerase AccuTaq™ LA DNA polymerasea LongAmp™ Taq DNA polymeraseb Crimson Taq™ DNA polymeraseb Phusion® High-Fidelity DNA Finally we would like to thank the following suppliers for DNA polym-erase samples to optimize the experimental condition upon; Fermentas DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. "The polymerase subunit of DNA polymerase III of Escherichia coli. II. Purification of the alpha subunit OmniAmp DNA polymerase (Pol) is a thermostable viral enzyme that enables true POC use in clinics or in the field by overcoming important barriers to Conventionally, LAMP uses Bst polymerase for amplification of DNA targets. In this paper, we report on applications and advantages of using Lower product yields with Pfu DNA polymerase have been attributed to the enzyme's relatively slow polymerization rate (550 vs. 2,800 nucleotides per min Improvements in the performance of Pfu DNA polymerase have been achieved by increasing PCR extension times (2 min/kb of target; Stratagene Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3? to 5? exonuclease proofreading activity, meaning that it works its way along the DNA from the 5? end to the 3? end and corrects nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments Taq DNA Polymerase is a highly thermostable DNA polymerase of a thermophilic bacterium •The error rate of Taq DNA Polymerase in PCR is 2.2x10-5 errors per nt per cycle; the accuracy (an 1.Innis, M.A., et al., PCR Protocols and Applications: A Laboratory Manual, Academic, New York, 1989. Taq DNA Polymerase is a highly thermostable DNA polymerase of a thermophilic bacterium •The error rate of Taq DNA Polymerase in PCR is 2.2x10-5 errors per nt per cycle; the accuracy (an 1.Innis, M.A., et al., PCR Protocols and Applications: A