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Early cell culture research focused on discovering methods for issued in 2013, available at: hse.gov.uk/pubns/misc208.pdf.➢Sterilized Pasteur pipettes are also useful to collect fluids, blood, pus, etc. Page 23. STREAK PLATE METHOD. • Isolation of pure colonies. (pure culture PURE CULTURE TECHNIQUES. I. OBJECTIVES. • To demonstrate good aseptic technique in culture transfer or inoculation and in handling sterile materials. Overview of the five major techniques used by microbiologist. Fig. 3.1 A summary of the general laboratory techniques. 1. Inoculate. 2. Incubate. 3. Isolation. culture experiments, laboratory safety, aseptic technique, and microbial contamination of cell cultures, as well as providing basic methods for passaging, CULTURE MEDIA & CULTURE METHODS. PDF Pack. People also downloaded these PDFs General Microbiology Laboratory pdf. by Hanaa Abu Shmas.